An approach towards surface imprinting using the enzyme ribonuclease A
โ Scribed by Maria Kempe; Magnus Glad; Klaus Mosbach
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 406 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0952-3499
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โฆ Synopsis
An adsorbent showing enhanced selectivity for the enzyme RNase A was prepared by a surface imprinting procedure based on metal coordination. A metal chelating monomer, N-(4-vinyl)-benzyl iminodiacetic acid, was polymerized onto methacrylate-derivatized silica particles in the presence of RNase A and metal ions. Lysozyme and RNase A were separated on the adsorbent used as stationary phase in high-performance liquid chromatography.
๐ SIMILAR VOLUMES
A stereo-and enantioselective approach towards the C 1 -C 16 fragment of bryostatins is reported using Jacobsen's catalyst for kinetic resolution of a terminal epoxide, a Horner-Wadsworth-Emmons coupling reaction and a 1,4-Michael type cyclization as key steps.