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An application of alumina column for the assay of adenine and hypoxanthine/guanine phosphoribosyltransferase activity: simple and rapid method

✍ Scribed by Kouichi Watanabe; Allen R. Rhoads; Evan F. Williams; Joseph S. Law; William L. West


Book ID
114074629
Publisher
Elsevier Science
Year
1978
Weight
700 KB
Volume
10
Category
Article
ISSN
0031-6989

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## Abstract A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H~2~O~2~ (HP), using a fluorescent europium–tetracycline (Eu~3~TC) complex as indicator. On addition of HP, this complex