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An analysis of the sequence of part of the right arm of chromosome II ofS. cerevisiaereveals new genes encoding an amino-acid permease and a carboxypeptidase

✍ Scribed by F. Nasr; A. -M. Bécam; E. Grzybowska; M. Zagulski; P. P. Slonimski; C J. Herbert

Book ID
104722937
Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
983 KB
Volume
26
Category
Article
ISSN
0172-8083

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✦ Synopsis

We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted membrane-spanning domains suggest a conserved structure for these proteins, with 12 trans-membrane domains. YBR1015 shows strong similarities to serine carboxypeptidases; all three residues of the "catalytic triad" typical of this family of enzymes are conserved in the YBR1015 protein. In a preliminary functional analysis we have created a null allele of the YBR1015 gene, and shown that it is not essential for cellular viability.

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