✦ LIBER ✦

A new technique of protein hydrolysis and analysis of the amino acids on an autoanalyzer

✍ Scribed by N. Maravalhas

Publisher: Elsevier Science
Year: 1970
Tongue: English
Weight: 516 KB
Volume: 50
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)97966-4

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✦ Synopsis

A new technique of protein hydrolysis is presented which has been shown to be more efficient than the classical HCl hydrolysis,

The new method consists in hydrolysis with crystallized oxalic acid with some added HCl in a sealed tube, The temperature of hydrolysis seems to be more critical than the time. After hydrolysis the frozen mass is dissolved, filtered, suitably diluted and chromatographed directly without neutralization. No damage to the columns was observed after fifty runs.

Only serine and threonine appear to suffer slight losses due to destruction and the arginine content was lowered somewhat. All the other amino acids show a better recovery.

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