An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos
β Scribed by D. Di Tuoro; M. Portaccio; M. Lepore; F. Arduini; D. Moscone; U. Bencivenga; D.G. Mita
- Book ID
- 113812718
- Publisher
- Elsevier
- Year
- 2011
- Tongue
- English
- Weight
- 397 KB
- Volume
- 29
- Category
- Article
- ISSN
- 1871-6784
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Aspects related to the use of free and immobilised acetylcholinesterase (AChE) for the development of an amperometric biosensor for organophosphorus (OP) insecticides based on enzyme inhibition have been studied. Procedures based on free and immobili~d AChE were previously studied from many authors
A stopped-flow/flow-injection procedure is proposed for the spectrophotometric determination of paraoxon based on the inhibition of immobilized acetylcholinesterase. The irreversibly inhibited enzyme is reactivated with l,l'-trimethylene-bi&-formylpyridinium bromide) dioxime. The proposed procedure
A highly sensitive flow analysis method for determination of acetylcholinesterase (AChE) inhibitors like organophosphorous pesticides using a new chemiluminescent reaction was developed and optimized. This method is fast, sensitive, and cheap, because it requires only one enzyme and its substrate. T