Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR-41 in batch and continuous culture

✍ Scribed by Guillermo R. Castro; Beatriz S. Méndez; Faustino Siñeriz

• Publisher: Wiley (John Wiley & Sons)
• Year: 2007
• Tongue: English
• Weight: 575 KB
• Volume: 56
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.280560312

✦ Synopsis

Bacillus arnyloliquefaciens MIR-41 has been isolated from natural sources and characterized using statistical methods. The strain showed three types of glucanase activities : a-amylase, a-glucosidase and pullulanase in defined medium and starch as carbon source. Extracellular activity of a-amylase was approximately three times higher in continuous culture than in batch. Maximal productivity was found at a dilution rate of 016 h-l. Alpha-glucosidase was expressed as a cell-bound enzyme in batch culture, but in continuous culture was mostly extracellular. Pullulanase was cell-bound in all cases. The expression of the enzymes was dependent upon the system and the dilution rate used. No cell lysis was detected in the chemostat as compared with batch cultures.