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Amyloid Proteins: Methods and Protocols

✍ Scribed by Einar M. Sigurdsson, Miguel Calero, María Gasset


Publisher
Humana Press
Year
2012
Tongue
English
Leaves
541
Series
Methods in Molecular Biology
Edition
2nd ed. 2012
Category
Library

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✦ Synopsis


Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word β€œamyloid” indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second editionΒ  of Amyloid Proteins expands upon the previous edition with current, detailed protocols for the preparation of amyloid and its precursors, specific analytical methods for studying these proteins, cell culture models and assays for production of amyloid proteins, and protocols for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. Written in the highly successful Methods in Molecular Biologyβ„’ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories. Authoritative and practical, Amyloid Proteins, Second Edition seeks to aid scientists in the amyloid field to establish new techniques in their laboratories.


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