Amyloid Proteins: Methods and Protocols
β Scribed by Gal Bitan, David B. Teplow (auth.), Einar M. Sigurdsson (eds.)
- Publisher
- Humana Press
- Year
- 2005
- Tongue
- English
- Leaves
- 407
- Series
- Methods in Molecular Biology 299
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
A proven collection of readily reproducible techniques for studying amyloid proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biologyβ’ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
β¦ Table of Contents
Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Front Matter....Pages 3-9
Front Matter....Pages 11-18
Front Matter....Pages 19-33
Back Matter....Pages 35-51
....Pages 53-65
β¦ Subjects
Biochemistry, general
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Amyloid diseases are characterized by the deposition of insoluble fibrous amyloid proteins. The word βamyloidβ indicates a starch-like compound, and though a misnomer, continues to be the accepted term for this group of protein conformational disorders. The second editionΒ of Amyloid Proteins expand
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