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Amyloid Proteins: Methods and Protocols

✍ Scribed by Gal Bitan, David B. Teplow (auth.), Einar M. Sigurdsson (eds.)


Publisher
Humana Press
Year
2005
Tongue
English
Leaves
407
Series
Methods in Molecular Biology 299
Edition
1
Category
Library

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✦ Synopsis


A proven collection of readily reproducible techniques for studying amyloid proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biologyβ„’ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

✦ Table of Contents


Front Matter....Pages i-xiv
Front Matter....Pages 1-1
Front Matter....Pages 3-9
Front Matter....Pages 11-18
Front Matter....Pages 19-33
Back Matter....Pages 35-51
....Pages 53-65

✦ Subjects


Biochemistry, general


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