Amplifications of DNA primase 1 (PRIM1) in human osteosarcoma

✍ Scribed by Wagner V. Yotov; Hugo Hamel; George-Etienne Rivard; Martin A. Champagne; Pierre A. Russo; Jean-Marie Leclerc; Mark L. Bernstein; Emile Levy

• Book ID: 101263517
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 133 KB
• Volume: 26
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199909)26:1<62::aid-gcc9>3.0.co;2-f

✦ Synopsis

We studied the involvement of PRIM1 in osteosarcoma by differential display, Northern and Southern hybridization, as well as fluorescence in situ hybridization (FISH) on interphase nuclei. In total, 22 pediatric oncology specimens were tested. PRIM1 was found to be amplified in 41% of the samples. PRIM1 is coamplified with the core 12q13 amplicon genes CDK4, SAS, and OS9, and was physically mapped very close to them. PRIM1 is therefore a new candidate for the role of a major target gene of 12q13 amplifications in human cancers.