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Amplification of unknown DNA sequences by sequence-independent nested polymerase chain reaction using a standardized adaptor without specific primers

✍ Scribed by H.-J. Schlayer; T. Peters; S. Preisler; J. Fehr; W. Gerok; J. Rasenack


Book ID
119115164
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
742 KB
Volume
38
Category
Article
ISSN
0166-0934

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We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only