Amplification of papillomaviral DNA sequences from a high proportion of feline cutaneous in situ and invasive squamous cell carcinomas using a nested polymerase chain reaction

Amplification and detection of a Y-chrom

Amplification and detection of a Y-chromosome DNA sequence by fluorescence in situ polymerase chain reaction and flow cytometry using cells in suspension

✍ Earl A. Timm Jr.; Ed Podniesinski; Carleton C. Stewart; Larry Duckett; John Card 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 554 KB

A procedure for amplifying and detecting nucleic acid sequences in situ using cells in suspension and flow cytometry has been developed. The process involves the use of the polymerase chain reaction (PCR) and a fluorescent in situ hybridization (FISH) protocol developed in our laboratory to detect t