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Amplification of circularizable probes for the detection of target nucleic acids and proteins

✍ Scribed by David Zhang; Josephine Wu; Fei Ye; Tao Feng; Ivy Lee; Bingjiao Yin


Book ID
116350293
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
169 KB
Volume
363
Category
Article
ISSN
0009-8981

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We have constructed light-up probes for nucleic acid detection. The light-up probe is a peptide nucleic acid (PNA) oligonucleotide to which the asymmetric cyanine dye thiazole orange (TO) is tethered. It combines the excellent hybridization properties of PNA and the large fluorescence enhancement of