A flow system for substrate recycling of NAD+/NADH was set up with an enzyme reactor containing coimmobiliied glycerol dehydrogenase (GDH) and diaphorase. The product from the diaphorase catalysis, hexacyanoferrate(II), was detected amperometrically at a glassy carbon electrode. The amplification fa
Amperometric enzyme electrode with the use of dehydrogenase and NAD(P)H oxidase
β Scribed by Fumio Mizutani; Soichi Yabuki; Tatsuo Katsura
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 161 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0925-4005
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AbStIBCt An amperometnc enzyme electrode for glucose was prepared by usmg a hpld-modlfied glucose ondase and a pexfluonnated monomer (Nafion) lkt, a glassy carbon base electrode was dipped mto a benzene solution of the modlfied enzyme and dned, then the electrode was dipped mto a Nafion solution and