Amperometric enzyme electrode with fast response to glucose using a layer of lipid-modified glucose oxidase and Nafion anionic polymer
✍ Scribed by Fumio Mizutani; Soichi Yabuki; Tatsuo Katsura
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 608 KB
- Volume
- 274
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
AbStIBCt An amperometnc enzyme electrode for glucose was prepared by usmg a hpld-modlfied glucose ondase and a pexfluonnated monomer (Nafion) lkt, a glassy carbon base electrode was dipped mto a benzene solution of the modlfied enzyme and dned, then the electrode was dipped mto a Nafion solution and dned The water-Insoluble, modlfied enzyme was thus mnnolxhzed on the electrode surface vnth a strongly adhenng, thm polymer coatmg The anodlc current (at 0 9 V vs Ag/AgCl) of this electrode Increased Imme&ately after the addltlon of glucose and the response time was shorter than 2 s A hnear response to glucose was observed up to 3 mM with a detectlon hnut of 0 2 PM The enzyme electrode was apphed to the determmatlon of glucose m fnut ounces, the Nafion coatmg was effectlve m suppressmg the electrochermcal Interference by t-ascorbate m the samples The electrode could be used for at least 6 weeks Keywords Amperometry, Enzymatic methods, Enzyme electrodes, Glucose AnalytIcal devices combmmg the specticlty of enzymatic reactions with high sensltlvlty of electrochenncal transduction have attracted mcreasmg interest m the last decade [1,2] Particular efforts m this field have been directed to the reahzatlon of an amperometnc sensor for the analytically significant substrate glucose by usmg glucose oxldase (GOD) [3] In conventional amperometrlc glucose-sensing electrodes, three membranes are attached on the surface of base electrode the first 1s used for protecting against foulmg of electrode, the second for munoblllzmg GOD and the third for ehmmatmg electroactrve mterferents Vanous approaches to developmg simple, rapid and reproducrble procedures for Correspondence to F &tam, National Institute of BIOscience and Human-Technology, l-l mgaslu, Tsukuba, lbaralo 305 (Japan)