𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Amperometric enzyme electrode for NADH detection employing co-immobilized lactate dehydrogenase and lactate oxidase

✍ Scribed by David A. Scott; Andrew W. Skillen

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
429 KB
Volume
256
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.

✦ Synopsis

A previously described method for the detectlon of NADH at platinum electrodes usmg the enzymes lactate dehydrogenase and lactate oxldase has been further developed and assessed The enzymes were co-lmmobdlzed on a single activated nylon membrane, which increased the sensltlvlty fourfold compared with the previous two-membrane electrode NADH was detected amperometrlcally with a hnear response m the concentration range 001-l 3 mM, v&h a slope of 280 nA 1 mmol-' The detectIon of NADH at 0 1, 10 or 2 0 mM was reproducible between membranes or usmg smgle membranes repeatedly Although the enzyme membranes steaddy lost actlvlty on storage, they mamtamed a hnear response to NADH concentrations up to 10 mM for up to 2 weeks Keywords Amperometry, Enzyme electrodes, NADH

📜 SIMILAR VOLUMES

Use of a biosensor consisting of an immo
Use of a biosensor consisting of an immobilized NADH oxidase column and a hydrogen peroxide electrode for the determination of serum lactate dehydrogenase activity
✍ Masayoshi Tabata; Fumiyo Koushima; Masayuki Totani 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 580 KB

A sensitive flow system for electrochemical determination of the reduced coenzyme NADH was developed using immobilized NADH oxidase from Buciflus lichenifomis. The immobilized enzyme was packed into a 20 mm x 2 mm I.D. mini-column, and the sample volume used was only 5 ~1 per assay, with an assay t