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Amperometric determination of cholesterol in serum with use of a renewable surface peroxidase electrode

✍ Scribed by L. Charpentier; N. El Murr

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
394 KB
Volume
318
Category
Article
ISSN
0003-2670

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✦ Synopsis

A sensitive modified carbon paste electrode for the direct determination of cholesterol is described. Both cyclic voltammetric and chronoamperometric techniques are used. The electrode was modified with hydroxymethyl ferrocene and horseradish peroxidase. Cholesterol oxidase and cholesterol ester hydrolase are used in solution. The measured current is linear with cholesterol concentration in the range 1 X 10e6 to 1.5 X low4 M. The measuring time is 30 s.

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