Amperometric Detection and Quantification of 8-Hydroxy-2′-deoxyguanosine (8-OHdG) Using Dendrimer Modified Electrodes

✍ Scribed by Alejandro Gutiérrez; Soraya Osegueda; Silvia Gutiérrez-Granados; Alejandro Alatorre; Ma. Guadalupe García; Luis A. Godínez

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 310 KB
• Volume: 20
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200804324

✦ Synopsis

Abstract

8‐Hydroxy‐2′‐deoxyguanosine (8‐OHdG) detection by high performance liquid chromatography (HPLC) with amperometric detection was studied using a Au electrode modified with different dendrimer based thin films. Gold electrode is thiol‐modified, forming self‐assembled monolayers on which different generation PAMAM dendrimers with terminal functional groups COOH and NH~2~ have been attached using peptidic bonds. Results obtained in synthetic samples show low limits of detection and quantification for 8‐OHdG (1.2×10^−9^ and 3.7×10^−9^ M respectively), with matrix interference elimination, thus avoiding sample pretreatment. Best results are obtained with electrodes modified with aliphatic amino thiols and 3.5 and 4.5 generation carboxylated dendrimers (Au/AET/DG^3.5^ and Au/AET/DG^4.5^), demonstrating that these materials constitute a good alternative for 8‐OHdG determination in biological fluids.