## Abstract Pure pencillin N α‐sulfoxide (**1**) and penicillin N β‐sulfoxide (**2**) were obtained by HPLC and tested as substrates for deacetoxycephalosporin C synthetase (DXCS). Neither one of the sulfoxides was utilized under conditions of conversion of penicillin N (**8**) to deacetoxycephalos
✦ LIBER ✦
Aminoglycoside antibiotics — two decades of their hplc bioanalysis
✍ Scribed by Ladislav Šoltés
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 76 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
Several reviews have been published on high-performance liquid chromatographic (HPLC) methods for the determination of aminoglycoside antibiotics (aminoglycosides) in biological fluids [e.g. Nilsson-Ehle, I. (1983).
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Yields of kinetically controlled synthesis of antibiotics catalyzed by penicillin G acylase from Escherichia coli (PGA) have been greatly increased by con- tinuous extraction of water soluble products (cephalexin) away from the surroundings of the enzyme. In this way its very rapid enzymatic hydroly