## Abstract To obtain a clearer concept of the mechanism of organic solute transport in mammalian cells, we have attempted to reconstitute a functional transport system for amino acids from plasma membranes of Ehrlich ascites cells. Purified plasma membranes were dissolved in 2% Na cholateβ4 M urea
Amino acid uptake in plasma membrane vesicles isolated from proliferating tumor cells and tissues
β Scribed by M. G. Leonardi; R. Comolli
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 433 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0939-4451
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Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried i n continuous spinner culture have been synchronized. Simultaneous monitoring of :tH-thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hours. This is composed o
## Abstract It has been reported previously that a plasma membraneβenriched fraction from 3T3 cells arrests the growth of sparse 3T3 cells early in the G~1~ phase of growth (Whittenberger et al., β²78, β²79). Addition of membranes to sparse 3T3 cells also results in a decrease of the rate of transpor