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Amino acid substitutions affecting protein solubility: high level expression of Streptomyces clavuligerus isopenicillin N synthase in Escherichia coli

โœ Scribed by Janet Sim; Tiow-Suan Sim


Book ID
114396371
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
109 KB
Volume
6
Category
Article
ISSN
1381-1177

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High-level soluble expression of isopeni
โœ J.E. Baldwin; J.M. Blackburn; J.D. Sutherland; M.C. Wright ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ French โš– 890 KB

The Isopenicillin N Synthase genes from Cephalosporium acremonium, Penicillium chrysogenum and Aspergillus nidulans have been expressed at high levels in Escherichia coli under control of the trc or lac promoters. The enzymes are produced in soluble form in contrast to previously reported expression