Compartmentalization of cellular amino acid pools occurs in cultures of cardiac and skeletal muscle cells, but the factors involved in this are not clear. We have further defined this problem by analyzing the intracellular free leucine and the transfer-RNA-(tRNA)-bound leucine pool in cultures of sk
Amino acid pools in mitotic and interphase HeLa cells
โ Scribed by J.C. Macmillan; D.N. Wheatley
- Book ID
- 118877791
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 655 KB
- Volume
- 133
- Category
- Article
- ISSN
- 0014-4827
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๐ SIMILAR VOLUMES
When the intracellular amino acid pool is prelabelled and subsequently chased in non-radioactive medium, the radioactivity of the amino acid pool is not found to have been incorporated into protein. (2) Leucine transport into Hela cells is reduced in the presence of 10 mM valine in the medium. This
## Abstract Mitotic HeLa cells (M cells) synthesize protein at about 25% of the rate of S phase cells. This decrease in protein synthesis is due to a reduction in the rate of initiation. However, extracts prepared from M cells are almost as active in protein synthesis as S cell extracts. Both cell