𝔖 Bobbio Scriptorium
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Amino acid pools in developing oocytes of Xenopus laevis

✍ Scribed by John J. Eppig Jr.; James N. Dumont

Book ID
118872598
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
457 KB
Volume
28
Category
Article
ISSN
0012-1606

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Development of a culture medium for grow
Development of a culture medium for growing xenopus laevis oocytes
✍ Wallace, Robin A. ;Misulovin, Ziva ;Jared, Donald W. ;Wiley, H. Steven πŸ“‚ Article πŸ“… 1978 πŸ› Wiley (John Wiley & Sons) βš– 785 KB

A defined medium for growing defolliculated Xenopus laevis oocytes was developed by monitoring vitellogenin incorporation. Optimum conditions were achieved by use of 50% Liebovitz L-15 medium supplemented with 1 mM L-glutamine, 15 mM Hepes, 5 mg/ml vitellogenin, and 1 pglmlinsulin (final pH = 7.8).