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Development of a culture medium for growing xenopus laevis oocytes

✍ Scribed by Wallace, Robin A. ;Misulovin, Ziva ;Jared, Donald W. ;Wiley, H. Steven

Publisher
Wiley (John Wiley & Sons)
Year
1978
Weight
785 KB
Volume
1
Category
Article
ISSN
0148-7280

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✦ Synopsis

A defined medium for growing defolliculated Xenopus laevis oocytes was developed by monitoring vitellogenin incorporation. Optimum conditions were achieved by use of 50% Liebovitz L-15 medium supplemented with 1 mM L-glutamine, 15 mM Hepes, 5 mg/ml vitellogenin, and 1 pglmlinsulin (final pH = 7.8). Stage IV/V oocytes remained morphologically normal in this medium for at least two weeks and grew at an average rate of 0.25 mm3 per week. This is the first time defolliculated Xenopus laevis oocytes have been grown in vitro.

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