## Abstract The capacity for synthesis of β‐alanine, sarcosine, and taurine was investigated in tissue slices from the little skate (__Raja erinacea__). Synthesis of β‐alanine was found to occur predominantly in the liver and at a rate comparable to the β‐alanine oxidation rate previously measured
Amino acid metabolism and cell volume regulation in the little skate,Raja erinacea. I. Oxidation
✍ Scribed by King, Patricia A. ;Cha, Chung-Ja ;Goldstein, Leon
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 745 KB
- Volume
- 212
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
The capacity of isolated tissues of the little skate (Raja erinacea) to oxidize β‐alanine, sarcosine, and taurine, and the role of amino acid oxidation in the modulation of intracellular free amino acid concentrations was investigated in vitro. Liver was found to be the primary site of β‐alanine and sarcosine oxidation. Chronic acclimation of skates to half‐strength sea water increased in vitro oxidation of β‐alanine and alanine by liver slices. Taurine oxidation could not be detected in any of the tissues tested. In vivo studies showed that taurine is excreted intact and that excretion increases significantly following acute acclimation of skates to half‐strength sea water.
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## Abstract Free amino acids comprise a significant fraction of the total intracellular osmolality in skate tissues. The high amino acid concentrations are maintained by active, Na^+^‐dependent transport systems in the cell membranes. Following transfer to a hypo‐osmotic environment (dilute seawate