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Amber dnaG mutation exerting a polar effect on the synthesis of RNA polymerase sigma factor in Escherichia coli

✍ Scribed by Nakamura, Yoshikazu

Publisher: Springer
Year: 1984
Tongue: English
Weight: 391 KB
Volume: 196
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00334114

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✦ Synopsis

Three amber mutants of Escherichia coli, dnaG9, dnaG24 and dnaG26, affected in the structural gene (dnaG) for "primase" have been isolated from a parental strain carrying a temperature-sensitive amber suppressor (supF-Ts6). These mutants grow at 30 degrees C but not at 42 degrees C since primase is essential for growth and is synthesized only at low temperatures. Chimeric plasmids carrying dnaG+ but no other chromosomal genes of E. coli complemented the amber mutations, and the plasmid carrying a part of dnaG lost the complementing activity. Beside, plasmids carrying a dnaG amber mutation complemented a temperature-sensitive dnaG mutation only in the presence of amber suppressor. One of the amber mutations, dnaG24 which maps proximal to the NH2-terminus of the dnaG gene, exerted a polar effect on the synthesis of RNA polymerase sigma factor in E. coli.

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