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Altered formation of DNA replication intermediates in cells growing in different culture conditions

✍ Scribed by Ulf lönn; Sigrid Lönn; Urban Nylen; Gerard Winblad

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
403 KB
Volume
138
Category
Article
ISSN
0021-9541

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✦ Synopsis

In human melanoma cells one can detect two discrete DNA replication intermediates, 1 0-kb DNA intermediates and Okakzaki fragments. Both intermediates are seen when cells are rapidly growing in medium supplemented with fetal calf serum. When the medium is supplemented with newborn calf serum, one can detect Okakzaki fragments but not 10-kb DNA intermediates. In contrast we do not detect changes in the replicon sizes in the two media.

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