Altered expression and transcription of the topoisomerase II gene in nitrogen mustard-resistant human cells
β Scribed by K.B. Tan; Steven R. Per; Rebecca A. Boyce; Christopher K. Mirabelli; Stanley T. Crooke
- Book ID
- 115780610
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 812 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0006-2952
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Raji-HN2 is a B cell lymphoma (Burkitt lymphoma) line that was made resistant to nitrogen mustard. The drug-resistant phenotype was accompanied by changes in gene expression. The expression of four unrelated genes was examined by Northern blot analysis. Raji-HN2 cells were found to contain about twi
Cellular resistance to etoposide has been correlated both with reduced levels and an aberrant cytoplasmic accumulation of the drug's target, topoisomerase IIβ£ (topo IIβ£). It is not known, however, whether a cytoplasmic pool of topo IIβ£ is sufficient to confer drug resistance on cultured mammalian ce