Alteration of tumorigenicity in undifferentiated thyroid carcinoma cells by introduction of normal chromosome 11

✍ Scribed by Akira Yoshida; Tarou Asaga; Chihiro Masuzawa; Satoru Kawahara

• Book ID: 102926363
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 744 KB
• Volume: 55
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930550308

✦ Synopsis

The tumorigenicity of various cell lines has been shown to be suppressed by the introduction of chromosome 11 and other chromosomes via microcell-mediated chromosome transfer. In this study, we investigated whether a human undifferentiated thyroid carcinoma cell line, TTA-1, was suppressed by the introduction of normal human chromosome 11 or 10. Chromosome 10 or 11 was transferred from A, cells containing a single human chromosome 10 or 11 tagged with pSV,-neo plasmid DNA into TTA-1 cells, by microcell fusion. The tumorigenicity of the TTA-1 cells and their colony-forming efficiency in soft agar were suppressed by chromosome 1 1 , but not by chromosome 10. These results suggest that normal human chromosome 11 carries a putative tumor suppressor gene that affects the tumor-associated phenotypes of TTA-1 cells.