Alteration of the cleavage mode and of the transglycosylation reactions of the xylanase A of Streptomyces lividans 1326 by site-directed mutagenesis of the Asn173 residue
✍ Scribed by Alain MOREAU; François SHARECK; Dieter KLUEPFEL; Rolf MOROSOLI
- Book ID
- 115130993
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 595 KB
- Volume
- 219
- Category
- Article
- ISSN
- 1432-1327
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
We attempted to expand the range of chlorinated solvents degraded by Xanthobacter autotrophicus GJ10 to include trichloroethylene by the rational modification of the enzyme haloalkane dehalogenase. The amino acids Phe164, Asp170, Phe172 and Trp175 were individually replaced with alanine by site-dire
## Abstract The α‐galactosidase (AGA) from __Bifidobacterium adolescentis__ DSM 20083 has a high transglycosylation activity. The optimal conditions for this activity are pH 8, and 37°C. At high melibiose concentration (600 mM), approximately 64% of the enzyme–substrate encounters resulted in trans