Alpha-1 antitrypsin response of stimulated alveolar macrophages

✍ Scribed by Zhi-An Yuan; Kenneth J. Soprano; Friedrich Kueppers

• Book ID: 102879819
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 590 KB
• Volume: 49
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240490411

✦ Synopsis

Alpha-I antitrypsin messenger RNA (AIAT mRNA) was determined in alveolar macrophages and in peripheral blood monocytes of healthy individuals using a sensitive RNase protection assay. Determinations were made of bacterial lipopolysaccharide (LPS) stimulated and unstimulated cells. We found that the amount of AlAT mRNA increased 7.3 and 14 times after 4 h of incubation with LPS for monocytes and macrophages, respectively (relative to total RNA). The increase was 12.3 and 14.8 times, respectively, when expressed as increase per cell. In both cell types there was wide interindividual variation in LPS response: 2-36 and 5-12 times for monocytes and macrophages, respectively.

The possible significance of A1 AT production of monocytes and macrophages may be the local control of granulocytic proteases such as elastase and cathepsin G.