Allelic losses at loci on chromosome 10 are associated with metastasis and progression of human prostate cancer

✍ Scribed by Akira Komiya; Hiroyoshi Suzuki; Takeshi Ueda; Ryuichi Yatani; Mitsuru Emi; Haruo Ito; Jun Shimazaki

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 733 KB
• Volume: 17
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199612)17:4<245::aid-gcc6>3.0.co;2-3

✦ Synopsis

DNA samples from tumors and paired normal tissues from 48 patients with prostate cancer (stage B, I 6 cases; stage C, I 4 cases; stage D, I 8 cases) were examined with 26 polymorphic markers spanning chromosome 10. Allelic losses were observed in I7 of the 46 cases (37%) that were informative with at least one of the markers. Detailed deletion mapping identified two distict commonly deleted regions on the long arm of chromosome I0 ( I Oq224247cM and I Oq25. I : I7cM) and one on I Op, suggesting that at least three tumor suppressor genes associated with prostate cancer are present on this chromosome. We observed loss of heterozygosity more frequently in tumors from fatal cases (stage D, 8/ 16, 50%) than in localized tumors (stage B, 01 16, 0%; P = 0.00 I or stage B + C, 5/30, 17%; P = 0.02 Fisher's exact test). All metastatic tissues showed allelic loss at one or more loci on I Oq. In five of the nine patients from whom DNAs were available from both metastatic and primary tumors, the primary cancer foci had no detectable abnormality of chromosome 10. while the metastatic foci showed allelic loss on chromosome 10.

These results suggested that inactivation of one or more tumor suppressor genes on chromosome 10 plays an important role in late stages of prostate cancer.