๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Alkylation of cysteine with acrylamide for protein sequence analysis

โœ Scribed by Daniel C. Brune


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
649 KB
Volume
207
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Identification of Cysteine Residues Alky
โœ R.A. Jue; J.E. Hale ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 479 KB

A new reagent for the routine identification of cysteine residues during protein sequencing is described. This method employs 3-bromopropylamine to alkylate cysteines in proteins after reduction with dithiothreitol. Upon sequencing of the protein on an Applied Biosystems 477 A protein sequencer, the

Determination of Cysteine Residues in Pr
โœ Reinout Amons ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 97 KB

HepG2 lysates. As expected, the highest signal was found for the liver cell line (24). The proposed assay is highly sensitive and allows screening of high numbers of samples in an easy and rapid way. All these characteristics together make the Determination of Cysteine Residues in Protein test very

Application of S-pyridylethylation of cy
โœ A.S. Mak; B.L. Jones ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 488 KB

Methods are given for determination of the phenylthiohydantoin derivative of pyridylethylcysteine (PTH-PECys) by gas chromatography, both with and without prior trimethylsilylation. Using the high-cysteine polypeptide purothionin, obtained from wheat, as a model system, cysteine residues at positio

Probing acrylamide alkylation sites in c
โœ Ellenia Bordini; Mahmoud Hamdan; Pier Giorgio Righetti ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 148 KB ๐Ÿ‘ 2 views

It is recognised that gel-separated proteins can experience a frequent modification provoked by the interaction of unpolymerized acrylamide monomers with the thiol group of cysteine to form a beta-cysteinyl-S-propionamide adduct. Other groups which have been implicated in this reaction include the h