A new reagent for the routine identification of cysteine residues during protein sequencing is described. This method employs 3-bromopropylamine to alkylate cysteines in proteins after reduction with dithiothreitol. Upon sequencing of the protein on an Applied Biosystems 477 A protein sequencer, the
On-Line Procedures for Alkylation of Cysteine Residues with 3-Bromopropylamine prior to Protein Sequence Analysis
โ Scribed by R.A. Jue; J.E. Hale
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 370 KB
- Volume
- 221
- Category
- Article
- ISSN
- 0003-2697
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