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Alkaline phosphatase synthesis in a cell-free system using DNA and RNA templates

โœ Scribed by Hiroshi Inouye; Charles Pratt; Jon Beckwith; Annamaria Torriani

Book ID
119443514
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
892 KB
Volume
110
Category
Article
ISSN
0022-2836

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Targeted nucleotide exchange in the alka
Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide
โœ B T Kren; A Cole-Strauss; E B Kmiec; C J Steer ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 597 KB

Although a variety of methods has been devised for modification of hepatic genes, none has been effective for long-term correction of genetic disorders. In this study, we employed a recently described novel experimental strategy for site-directed nucleotide exchange in genomic DNA of HuH-7 human hep