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A simple and cost-effective method to prepare template DNAs for cell-free protein synthesis

โœ Scribed by Ju-Young Byun; Kyungmoon Park; Dong-Myung Kim


Book ID
107514353
Publisher
Springer US
Year
2010
Tongue
English
Weight
484 KB
Volume
27
Category
Article
ISSN
0256-1115

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โœ Thomas E. Ferrari; Jack M. Widholm ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 390 KB

A simple, rapid, and sensitive method for the measurement of DNA, RNA, and protein synthesis in cell samples is presented. Tke method measures the amount of isotope incorporated into these macromolecules after cell collection on filter paper and washing with a methanol:chloroform:water mixture. Samp