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Alkaline Phosphatase Revisited: Hydrolysis of Alkyl Phosphates †

✍ Scribed by O'Brie, Patrick J.; Herschlag, Daniel


Book ID
121421445
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
215 KB
Volume
41
Category
Article
ISSN
0006-2960

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✍ Harborn, Stuart ;Eggelte, Henny J. ;Benson, Suzanne M. ;Rabin, Brain R. 📂 Article 📅 1991 🏛 John Wiley and Sons ⚖ 488 KB

An asasy for alkaline phosphatase is described which is based on the hydrolysis of riboflavin phosphates (5'FMN or 4 F M N ) t o produce riboflavin. This is converted t o 5'FMN using riboflavin kinase. and then asayed using the bacterial bioluminescent system from Vibrio harveyi or V. fischeri. The