There has been a considerable and increasing interest in the phosphatase problem for more than 15 years, as evidenced by the great number of papers published on it throughout the world. I n 1939 two practically identical methods for the demonstration of phosphatase in microscopic sections were devel
Alkaline phosphatase and its isoenzymes in the tissues and sera of normal dogs
β Scribed by Elspeth M. Milne; D. L. Doxey
- Publisher
- Springer Netherlands
- Year
- 1986
- Tongue
- English
- Weight
- 336 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0165-7380
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HIS REPORT deals with the histochemical T demonstration of alkaline phosphatase activity in tissue sections of 87 benign and malignant tumors and various inflammatory gastrointestinal diseases. Alkaline phosphatase was demonstrated with a simultaneous azo dye coupling method. ## Methods Represent
Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4 degrees C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning