✦ LIBER ✦

Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased

✍ Scribed by L. Bell; L. Williams

Publisher: Springer
Year: 1979
Tongue: English
Weight: 842 KB
Volume: 60
Category: Article
ISSN: 1432-119X
DOI: 10.1007/bf00495731

No coin nor oath required. For personal study only.

✦ Synopsis

Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4 degrees C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 mu, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 mu. The simultaneous coupling azo dye method using the substrates sodium alpha-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.

📜 SIMILAR VOLUMES

Demonstration of placental alkaline phos

Demonstration of placental alkaline phosphatase in human breast cancer

✍ H. Garrett Wada; Jeffrey E. Shindelman; Ann E. Ortmeyer; Howard H. Sussman 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 635 KB

## Abstract The screening of a series of 11 metastatic breast tumors for the presence of the placental isoenzyme of alkaline phosphatase (EC 3.1.3.1) by RIA revealed one strong producer. The alkaline phosphatase of this tumor was characterized with respect to its immunochemical cross‐reactivity, in

[3H]Thymidine autoradiographic and alkal

[3H]Thymidine autoradiographic and alkaline phosphatase histochemical studies of intestinal metaplasia of the human stomach

✍ Hashimoto, Masayoshi ;Tokunaga, Akira ;Nishi, Keigo ;Wada, Masayo ;Masumori, Koj 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 718 KB

Large-scale gel-filtration in the purifi

Large-scale gel-filtration in the purification of human liver and small intestine alkaline phosphatases

✍ J.K. Smith; R.Helen Eaton; L.G. Whitby; D.W. Moss 📂 Article 📅 1968 🏛 Elsevier Science 🌐 English ⚖ 702 KB

Chemical and histochemical studies of no

Chemical and histochemical studies of normal and diseased human gastrointestinal tract. III. Changes in the histochemical and chemical properties of the epithelial glycoproteins in the mucosa close to colonic tumours

✍ Reid, P. E. ;Owen, D. A. ;Dunn, W. L. ;Ramey, C. W. ;Lazosky, D. A. ;Clay, M. G. 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 750 KB

Expression profiling of Wnt family of ge

Expression profiling of Wnt family of genes in normal and inflammatory bowel disease primary human intestinal myofibroblasts and normal human colonic crypt epithelial cells

✍ K.R. Hughes; F. Sablitzky; Y.R. Mahida 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 169 KB 👁 2 views

Background: Wnt signaling regulates intestinal epithelial stem cell function. Wnt ligands bind Frizzled (Fz) receptors and lowdensity lipoprotein-receptor-related protein (LRP) 5 and 6. Secreted Frizzled-related protein (SFRP) and Dickkopf families inhibit Wnt signaling. Our aim was to study express