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Alkaline hydrolysis of and effect of heat on ribonucleic acid

Publisher
Elsevier Science
Year
1946
Tongue
English
Weight
390 KB
Volume
242
Category
Article
ISSN
0016-0032

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โœฆ Synopsis

Alkaline Hydrolysis of and Effect of Heat on Ribonucleic Acid.-CHARLES A. ZITTLE. Variation in the ease of hydrolysis of different samples of ribonucleic acid with alkali has been observed (I, 2, 3). Since an aged sample of nucleic acid had become more difficult to hydrolyze (I), the effect of heat on nucleic acid was investigated as the most likely cause of the variations in hydrolyzability. The reactivity of the heated nucleic acids with the phosphodiesterase ribonucleinase and with a less specific phosphodiesterase (4) was studied as well. From these and other experiments in which the concentration of NaOH was varied it was concluded that the concentration of NaOH was critical for the hydrolysis of ribonucleic acid to mononucleotides. In addition to this stage of hydrolysis of the nucleic acid, measured by precipitation with the uranium reagent (decomposition of tetranucleotides), another stage was evident by the decrease in pH of the hydrolysates which continued when the former had ceased, and it, too, was characterized by a critical pH requirement.

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