๐”– Bobbio Scriptorium
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Alkali enhancement of protein staining on nitrocellulose

โœ Scribed by Mark W. Sutherland; John H. Skerritt

Book ID
102834542
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
721 KB
Volume
7
Category
Article
ISSN
0173-0835

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โœฆ Synopsis

North Ryde

Brief alkali treatment of nitrocellulose-bound protein markedly enhanced subsequent staining of protein with India ink or colloidal gold but not Amido Black. Following alkali treatment, India ink staining approaches colloidal gold staining in sensitivity and exceeds the latter method in clarity and economy. Furthermore, apparent protein-to-protein variation is reduced, allowing the method to be used for sensitive staining of protein transfers. Simple, high sensitivity staining of nitrocellulose-bound protein using India ink can be achieved after alkali treatment in less than 2 hours.

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