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Alcohol oxidation by Acinetobacter calcoaceticus EB 104 — a n-alkane-utilizing and cytochrome P-450-producing strain

✍ Scribed by M. Jirausch; O. Asperger; H.-P. Kleber

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 505 KB
Volume: 26
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620260611

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✦ Synopsis

A soluble, NADP+-dependent alcohol dehydrogenase (ADH) with a pH optimum at 8.7 was found in A. calcoaceticus EB 104 after growth on different carbon sources. n-Alkanols with short and medium chain length were employed as test substrates. The Km values decreased with increasing chain length. The Vmax values remained nearly unchanged. The activities determined were independent of the carbon source. Furthermore, a n-alkanol-dependent reduction of DCPIP was measured in membrane fractions of cells grown on different carbon sources. The optimum pH for this reaction was at 7.5. Further proof for the presence of a pyridine nucleotide-independent ADH was derived from the oxidation of 14C-decanol in the absence of NADP+ or NAD+.

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Effect of oxygen Iimitation on the content of n-hexadecane-inducible cytochrome P-450 in Acinetobacter calcoaceticus strain EB 104

✍ A. A. Sharychev; R. N. Matyashova; A. B. Losinov; Dr. O. Asperger; H.-P. Kleber 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 415 KB

The content of cytochrome P-450 as a function of oxygen supply was studied during growth of Acinetobacter on n-hexadecane in batch cultures at constant p H and agitation. The rate of growth and the content of cytochrome P-450 were not affected as long as the dissolved oxygen tension ranged above 3 t