Effect of oxygen Iimitation on the content of n-hexadecane-inducible cytochrome P-450 in Acinetobacter calcoaceticus strain EB 104
✍ Scribed by A. A. Sharychev; R. N. Matyashova; A. B. Losinov; Dr. O. Asperger; H.-P. Kleber
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 415 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
The content of cytochrome P-450 as a function of oxygen supply was studied during growth of Acinetobacter on n-hexadecane in batch cultures at constant p H and agitation. The rate of growth and the content of cytochrome P-450 were not affected as long as the dissolved oxygen tension ranged above 3 t o 5% of saturation. The amount of cytochrome P-450 increased when the oxygen tension declined to zero. Cytochrome P-450 levels of about 0.3 to 0.4 nmol/mg protein, i.e. a more than a threefold increase, were observed under conditions where oxygen supply was strictly limited and allowed to maintain only a minimum of metabolism or growth. Limited oxygen supply exerted a special effect on the induction of the cytochrome P-450 as concluded from an increasing ratio between cytochrome P-450 and cytochrome 0, and from the absence of cytochrome d in cells with elevated content of cytochrome P-450. The increased formation of cytochrome P-450 was a reversible process.