Agrobacterium rhizogenes-mediated DNA transfer inPinus halepensisMill.
β Scribed by Tzvi Tzfira, Ophir Yarnitzky, Alexander Vainstein, Arie Altman
- Book ID
- 120779734
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 735 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0721-7714
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DNA transfer using Agrobacterium tumefaciens has been demonstrated in sugar pine, Pinus lambertiana Dougl. Shoots derived from cytokinin-treated cotyledons formed galls after inoculation with A. tumefaciens strains containing the plasmid pTiBo542. A selectable marker, neomycin phosphotransferase II,
Axenic root tissue cultures were established from primary hairy roots induced on carrot and potato by Agrobacterium rhizogenes strain 15834. cDNA made towards poly-A + RNA isolated from these tissues, hybridized with a limited number of well-defined fragments of the plasmid DNA present in the inciti