DNA from Agrobacterium rhizogenes in transferred to and expressed in axenic hairy root plant tissues
✍ Scribed by Willmitzer, Lothar ;Sanchez-Serrano, José ;Buschfeld, Ellen ;Schell, Jeff
- Book ID
- 104769333
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 856 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Axenic root tissue cultures were established from primary hairy roots induced on carrot and potato by Agrobacterium rhizogenes strain 15834. cDNA made towards poly-A + RNA isolated from these tissues, hybridized with a limited number of well-defined fragments of the plasmid DNA present in the inciting A. rhizogenes strain. These data therefore demonstrate that at least part of the rootinducing (Ri) plasmid of Agrobacterium rhizogenes is transferred, stably maintained and expressed in hairy-root plant tissues and confirm that hairy roots are a special type of crown gall. The T-DNA in hairy-root cells appears to have several regions which are related in terms of sequence homology and probably also function to the T-DNA in octopine and nopaline crown gall tumours.