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Agitation and aeration effects in suspension mammalian cell cultures

✍ Scribed by C. P. Marquis; K. S. Low; J. P. Barford; C. Harbour

Publisher
Springer
Year
1989
Tongue
English
Weight
641 KB
Volume
2
Category
Article
ISSN
0920-9069

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✦ Synopsis

Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m-2. Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effects at the reactor level have been included here. A discussion of the difference between suspension and microcarrier cultures, in relation to hydrodynamic effects, is included.

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