Aggregation and fusion of plant protoplasts after surface-labelling with biotin and avidin
β Scribed by W.J.P. van Kesteren; E. Molema; M.J. Tempelaar
- Book ID
- 119137042
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 900 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0168-9452
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## Abstract Surface labelling of plant protoplasts was tested for use in mass fusion systems and heterokaryon detection. Parameters have been established for biotinylation and subsequent incubation with avidinβcoupled fluorochromes. The procedure is rapid (less than 3 hours) and does not affect via
Multilayer membranes composed of avidin and biotin-labeled glucose oxidase (B-GOD) or of an avidin-B-GOD complex and B-GOD were prepared on an indium tin oxide (ITO) electrode, based on the high affinity between avidin and B-GOD. The B-GOD-modified ITO electrodes can be used to determine glucose ele