𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Agglutination assay of lectins in presence of specific sugars by using borate

✍ Scribed by Renu Tyagi; M. N. Gupta

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
204 KB
Volume
7
Category
Article
ISSN
0951-208X

No coin nor oath required. For personal study only.

✦ Synopsis

Incorporation of borate anion allows the agglutination activity of a lectin to be observed even in the presence of the specific sugar. This simple, convenient procedure worked successfully with five different lectins with different sugar specificities.

πŸ“œ SIMILAR VOLUMES

Determination of the Sialic Acid Linkage
Determination of the Sialic Acid Linkage Specificity of Sialidases Using Lectins in a Solid Phase Assay
✍ F. Rogerieux; M. Belaise; H. Terzidistrabelsi; A. Greffard; Y. Pilatte; C.R. Lam πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 431 KB

A procedure for the determination of activity and linkage specificity of sialidases is deseribed. The sialoglycoprotein fetuin is coated onto a microtiter plate and incubated with sialidases from different sources. Enzymatic activities and linkage specificities are then determined by a sandwich meth

Assessment of lectin activity in a toxic
Assessment of lectin activity in a toxic and a non-toxic variety ofJatropha curcas using latex agglutination and haemagglutination methods and inactivation of lectin by heat treatments
✍ Aregheore, Eroarome M; Makkar, Harinder P S; Becker, Klaus πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 178 KB πŸ‘ 1 views

Lectin activity in a toxic (Cape Verde) and non-toxic (Mexico) variety of Jatropha curcas seed meal was investigated using latex agglutination and haemagglutination assays. Lectin activity expressed as reciprocal of the minimum quantity (in mg) of Jatropha meal per ml of the assay mixture which prod

Enhanced Firefly Bioluminescence Assay o
Enhanced Firefly Bioluminescence Assay of ATP in the Presence of ATP Extractants by Using Diethylaminoethyl-Dextran
✍ Akihiko Ishida; Takahiro Yoshikawa; Takatoshi Nakazawa; Tamio Kamidate πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 69 KB

A highly sensitive ATP bioluminescence assay with diethylaminoethyl-dextran (DEAE-Dx) in the presence of ATP extractants such as trichloroacetic acid (TCA) and Triton X-100 is described. These ATP extractants inhibited the activity of firefly luciferase, resulting in a remarkable decrease in the int

Detection of early antigens in nuclei of
Detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity
✍ G. Giraldo; E. Beth; U. HΓ€mmerling; G. Tarro; F. M. Kourilsky πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 French βš– 975 KB

## Abstract Skin fibroblasts exposed to cytosine arabinoside (Ara C) were infected with either cytomegalovirus (CMV) or herpes simplex virus (HSV) type 1 and 2. Herpesvirus‐determined early antigens (HV‐EA), detected by anti‐complement immunofluorescence (ACIF), occurred primarily in the nuclei, an