Assessment of lectin activity in a toxic and a non-toxic variety ofJatropha curcas using latex agglutination and haemagglutination methods and inactivation of lectin by heat treatments
✍ Scribed by Aregheore, Eroarome M; Makkar, Harinder P S; Becker, Klaus
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 178 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Lectin activity in a toxic (Cape Verde) and non-toxic (Mexico) variety of Jatropha curcas seed meal was investigated using latex agglutination and haemagglutination assays. Lectin activity expressed as reciprocal of the minimum quantity (in mg) of Jatropha meal per ml of the assay mixture which produced agglutination with the latex beads was 2É88 ^0É57 and 1É71 ^0É00 (mean ^SD, n \ 3) for the toxic and the non-toxic varieties, respectively, which did not di †er signiÐcantly (P [ 0É05), while with the haemagglutination assay these values were 102 and 51, respectively, and di †ered signiÐcantly (P \ 0É05). The lectins were inactivated by heating and moist heat was more e †ective than dry heat. The results suggest that lectins may not be responsible for short term toxicity caused by consumption of raw Jatropha meal.
1998 SCI.