Adult rat hepatocytes formed spherical multicellular aggregates (spheroids) when they were cultured in the pores of polyurethane foam (PUF). The diameter of the spheroids was within the range 100-200 microns. These spheroids partly attached and immobilized in the PUF pores for at least 2 weeks. The
Agar coating in multicellular spheroids culture of rat hepatocytes
โ Scribed by Shaolin Li; Jilun Pan; Tao Xu; Yaoting Yu
- Book ID
- 105642523
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 289 KB
- Volume
- 44
- Category
- Article
- ISSN
- 1001-6538
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Adult rat hepatocytes in primary culture formed floating, non-spheroidal aggregates on the surfaces coated with 1 % polyacrylamide. In the cells of aggregates, DNA synthetic capacity was limited, but albumin secreting capacity was maintained at least for 2 weeks. These properties were influenced by
We designed a bioartificial liver support system in which encapsulated multicellular spheroids of rat hepatocytes were utilized as a bioreactor in a hollow fiber cartridge. The spheroids, formed in a positively charged polystyrene dish that contained hormonally defined medium, were encapsulated into