When using weakly interacting ligands in affinity chromatography, it is possible to take advantage of a true chromatographic process in the separation, as compared with traditional affinity chromatography which is rather an on/off process. In this work, weak monoclonal antibodies were immobilized on
Affinity screening for weak monoclonal antibodies
β Scribed by Lisa Leickt; Anders Grubb; Sten Ohlson
- Book ID
- 114052086
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 105 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0022-1759
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
As the interest in weak-affinity antibodies has been widened by their introduction to various analytical techniques such as HPLC, capillary electrophoresis and biosensors, there has been a need for new screening/ monitoring methods. In this study, weak-affinity chromatography was adopted to screen/m
## Affinity electrophoresis with monoclonal antibodies Affinity electrophoresis was applied to the study of binding of monoclonal antibodies (MoAb's) with their respective antigens. The MoAb's may be either mixed with the antigen in an application well or incorporated into the agarose gel. The fir